missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Réactifs pour l’analyse des protéines
  4. Lysats
  5. Lysats de lignées cellulaires
  6. 16089615

SKOV-3 (human adenocarcinoma) Whole cell lysate, Denatured; Abnova

Code produit 16089615
Modifier l'affichage
Click to view available options
Quantité:
200 μg
Conditionnement:
200µg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Code produit Quantité Conditionnement
16089615 200 μg 200µg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Les retours ne sont pas autorisés pour ce produit. Afficher la politique du retour.
Code produit 16089615 Fournisseur Abnova Code fournisseur L048V1.200ug
 Article restreint
Expédition estimée: 01-12-2026
Ajouter au panier
Ajouter au panier

missing translation for 'validMainframeMsg'

Les retours ne sont pas autorisés pour ce produit. Afficher la politique du retour.

Description

Transfert Western

Spécification

À utiliser avec (application) Western Blot
Tissue Ovary
Description Whole cell lysate (denatured)
Tampon de lyse Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Méthode de préparation Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Test du contrôle qualité 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantité 200 μg
Tampon de stockage In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Espèces hôtes Human
Concentration 3 mg/mL
Contenu et stockage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Show More Show Less
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.