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Applied Biosystems™ Dosages du nombre de copies TaqMan™
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Description
Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNA targets using Applied Biosystems real-time PCR instruments and software. TaqMan Copy Number Assays are run together with a TaqMan Copy Number Reference Assay in a duplex qPCR reaction; the copy number assay detects the target sequence, and the reference assay detects a sequence that is known to be present in two copies in the diploid genome. The results are then analyzed by the relative quantitation method using Applied Biosystems CopyCaller Software.
Specifications
Specifications
| Modification de l’amorce de 3’ | Néant |
| Modification de l’amorce de 5’ | Néant |
| Concentration | 20X |
| Contenu et stockage | 1 tube contenant un mélange de dosage préformulé 20X (tailles S et M) ou 60X (taille L) (1 sonde et 2 amorces). Conserver entre -15 et -25°C. |
| Méthode de détection | Amorce-sonde |
| Forme | Congelé |
| Méthode PCR | qPCR |
| Vitesse de réaction | Rapide |
| À utiliser avec (équipement) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System |
| Modification interne de la sonde | FAM (5’), MGB (Minor Groove Binder) (3’) |
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Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
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