Invitrogen™ EPO Receptor Human ELISA Kit

Description

The Human EPO Receptor (EPO R) ELISA quantitates Hu EPO R in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu EPO R. Principle of the method The Human EPO R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Le récepteur de l’érythropoïétine humaine recombinante (EPO R) est une protéine transmembranaire de type I, exprimée dans une lignée cellulaire de myélome de souris, NSO. Ce produit est produit à partir d’une séquence d’ADNc codant le domaine extracellulaire du récepteur de l’EPO. Le récepteur de l’EPO soluble recombinant à maturité, à 225 acides aminés, généré après clivage d’un peptide signal de résidus de 25 acides aminés, a prédit une masse moléculaire d’environ 25 kDa.