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Gibco™ Human HGF Recombinant Protein

Código de producto. p-7075952
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Quantité:
10 μg
100 μg
Tamaño de la unidad:
100µg
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Código de producto. 12073599

Marca: Gibco™ PHG0321

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Descripción

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile PBS containing 0.1% human serum albumin. Apportion the reconstituted protein into working aliquots and store at = -20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA. Storage: Store the lyophilized protein at = -20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at = -20°C. Avoid repeated freeze-thaw cycles.

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.
TRUSTED_SUSTAINABILITY

Especificaciones

Numéro d’adhésion P14210, Q08048
À utiliser avec (application) Bioactivity
Formule PBS with no preservative
Identification génétique (Entrez) 15234, 3082
Poids moléculaire 70 kDa
Nom Human HGF
Méthode de purification Purified
Quantité 100 μg
Conditions de stockage -20°C
État réglementaire RUO
Concentration en endotoxines <0.15 EU/μg
Alias de gène C230052L06Rik; DFNB39; Fibroblast derived tumor cytotoxic factor; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor; Hepatocyte growth factor (hepapoietin A; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; Hepatopoeitin A; hepatopoeitin-A; HepatopoietinA; hepatopoietin-A; Hgf; HGF/SF; HGFB; HPTA; Lung fibroblast derived mitogen; lung fibroblast-derived mitogen; NK1; NK2; Scatter factor; scatter factor); SF; SF/HGF
Nom usuel HGF
Symbole de gène(s) Hgf
Activité biologique ED50 ≤ 20 ng/mL; determined by the dose-dependent proliferation of 4 MBr-5 cells.
Type de produit Protein
Conjugué Unconjugated
Espèces Human
Recombinant Recombinant
Contenu et stockage -20°C
Classification Carrier-Free
Activité ED50 ≤ 20 ng/mL; determined by the dose-dependent proliferation of 4MBr-5 cells.
Dosage biologique Proliferation of the monkey epithelial cell line 4 MBr-5
Niveau d’endotoxines <1 EU/ml
Conditions d’expédition Dry Ice
Système d’expression HEK293 cells
Famille de protéines Growth Factors & Receptors
Forme protéique Recombinant, Ligand
Forme Lyophilized
Sous-type de protéine HGF (Hepatocyte Growth Factor)
Catégorie de recherche Cell Cycle & Proliferation, Oncology, Stem Cell Research, Signal Transduction, Angiogenesis
Pureté ou qualité >95% by SDS-PAGE
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