Invitrogen™ Kit ELISA™ Instant de RANTES humain

Includes

• Sachet(s) en aluminium avec microplaque revêtue d’anticorps monoclonal (murine) contre le RANTES humain, conjugué à la biotine (anticorps polyclonal contre le RANTES), diluant d’échantillon et streptavidine-HRP, lyophilisé
• Sachet(s) en aluminium avec courbe étalon RANTES humain (colorée)
• Concentrat de tampon de lavage 20 x (solution saline tamponnée au phosphate avec 1 % de Tween 20)
• Solution de substrat (tétraméthyl-benzidine)
• Concentrat de tampon de dosage 20x (PBS avec 1 % de Tween 20 et 10 % de BSA)
• Solution d’arrêt (Acide phosphoreux 1M)
• Couvercles de plaques adhésives

Kit ELISA instantané

The Human RANTES(Hu regulated upon activation, normal T-cell expressed and secreted)) ELISA quantitates Hu RANTES in human serum, plasma, cell culture supernatants, amniotic fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu RANTES. Principle of the method The Human RANTES solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.