Invitrogen™ Kit ELISA™ Instant de récepteur d’IL-6 (soluble) humain

Includes

• Sachet(s) en aluminium avec microplaque revêtue d’anticorps monoclonal (murin) contre le sIL-6R humain et conjugué à la HRP (anticorps monoclonal [murin] dirigé contre le sIL-6R), lyophilisé
• Sachet(s) en aluminium avec courbe étalon de sIL-6R humain (colorée)
• Concentrat de tampon de lavage 20x (solution saline avec tampon phosphate avec 1 % de Tween 20)
• Solution de substrat (tétraméthyle-benzidine)
• Concentrat de tampon de dosage 20x (PBS avec 1 % de Tween 20 et 10 % de BSA
• Solution d’arrêt (acide phosphorique 1M)
• Contrôle lyophilisé
• Couvercles de plaque adhésive

Kit ELISA instantané

The Human Soluble Interleukin 6 Receptor(Hu sIL-6R) ELISA quantitates Hu sIL-6R in human serum, plasma, cell culture supernatants, urine, amniotic fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sIL-6R. Principle of the method The Human sICAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.