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Jurkat, clone E6-1 (human acute T cell leukemia) Whole cell lysate, Denatured; Abnova
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Quantité:
200 μg
Conditionnement:
200µg
Description
- Tissue: T Lymphoblast
- Host: Human
- Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
- Storage buffer: In ready-to-use 1X Sample Buffer (50mM Tris-HCl, 2% SDS, 10% glycerol, 300mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Western Blotting
Spécification
Spécification
| À utiliser avec (application) | Western Blot |
| Tissue | T Lymphoblast |
| Description | Whole cell lysate (denatured) |
| Tampon de lyse | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Méthode de préparation | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly. |
| Test du contrôle qualité | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Quantité | 200 μg |
| Tampon de stockage | In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue). |
| Espèces hôtes | Human |
| Contenu et stockage | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
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