Invitrogen™ Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight 594

The tomato lectin LEL is widely used as marker of blood vessels and microglial cells in rodents and in neuroscience research. It is a valuable tool in studies of rodent tumor angiogenesis and the tracing of neovascular development in xenograft models and brain research. LEL-DyLight 594 can be applied directly to tissue sections, or it can be introduced through intravascular perfusion for fluorescence detection.

• Very bright DyLight 594 conjugate detected with traditional Texas Red filter set, Ex/Em: 592/617 nm
• Applications: immunofluorescence, glycobiology
• Sugar specificity: [GlcNAc] 1,3-N-acetylglucosamine
• Inhibiting/eluting sugar: chitin hydrolysate
• Blood group specificity: non-specific
• Unconjugated lectin molecular weight: 71 KDa.
• Immunofluorescence working concentration: 5–20 μg/mL

LEL is a very stable single-subunit glycoprotein containing ∼50 percent arabinose and galactose and may form multimeric aggregates in solution. It is provided at a 1 mg/mL concentration in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca²⁺. If a precipitate forms upon long-term storage, warm to 37°C.