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Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Description
Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.
Features of Lyo-ready RPA products
- Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
- Robust and specific RNA/DNA amplification
- Amplification from inhibitor-containing samples
- Fast target amplification in as little as 20 minutes
- Ability to easily optimize RPA or RT-RPA reactions
Kit components
The Lyo-ready RPA Kit includes all required components for an RPA reaction:
- Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
- Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
- Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
- Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
- Optimized reaction buffer, magnesium chloride, and dNTPs
Spécification
Spécification
| Type de produit | Tampon de réaction |
| Forme | Liquid |
| À utiliser avec (application) | RPA, RT-RPA |
| Quantité | 2 000 μl |
| Catégorie de recherche | Nucleic Acid Amplification |
| Contenu et stockage | 2X RPA Reaction Buffer, 2 mL, Store at -25°C to -15°C. |
| Température de réaction optimale | 42°C |
| Conditions d’expédition | Dry Ice |
| Concentration | 2X |
| Temps de réaction | Up to 20 min. |
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
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