Invitrogen™ ELISA Ready-SET-Go!™ IL-17 AF pour souris (hétérodimère) eBioscience™ Kit, 2e génération

Includes

• Anticorps de capture :Anticorps purifié, prétitré
• Anticorps de détection.Anticorps conjugué à la biotine, prétitré
• Étalon.Cytokine recombinante pour générer une courbe standard et étalonner des échantillons
• Tampon de revêtement 10X PBS Tampon de revêtement ELISA.
• Diluant de dosage.Concentré 5X
• Diluant d’échantillon A
• Enzyme de détection.Avidine-HRP prétitrée
• Solution de substrat.Solution de substrat de tétraméthylbenzidine (TMB)

Spécialement conçu pour une mesure précise et exacte des niveaux de protéines à partir d’échantillons, y compris les surnageants de culture cellulaire et de sérum.

The Mouse Interleukin 17AF (heterodimer) (IL-17AF) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-17AF(heterodimer). Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.