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Invitrogen™ Novex™ DYNAL™ Dynabeads™ Co-Immunoprecipitation Kit

Allows easy coupling of an antibody to the surface of uniform, 2.8μm super paramagnetic Dynabeads M-270 Epoxy beads

Marque:  Invitrogen™ 14321D

Code nomenclature Nacres: NA.28

Informations supplémentaires : Poids : 0.66000kg

Code produit. 10365438

  • 612.00€ / 1 pièce


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Includes: Dynabeads™ M-270 Epoxy (>60mg freeze-dried), Buffer C1 (20mL), Buffer C2 (8mL), Buffer HB (15mL), Buffer LB (15mL), Buffer SB (40mL), 5X IP Buffer (120mL), 5X LWB (24mL), Buffer EB (4mL)

Description

Description

Kit is designed for and tested with cultured yeast and mammalian cells and is compatible with tissue, insect, bacteria, and othe lysates. Following immobilization of antibody, use coupled beads and buffers for co-immunoprecipitation (co-IP) of proteins, intact protein complexes, or intact protein-nucleic acid complexes and to elute those complexes from the beads. In addition to Dynabeads, the kit supplies the buffers needed for efficient antibody coupling, gentle washing, and elution of the co-isolated protein complexes.

  • Optimized buffers and epoxy-coated Dynabeads for efficient co-IP included in the kit
  • Gentle separation technology: preserves native protein conformations and large-protein complexes
  • Covalent antibody coupling to the Dynabeads avoids co-elution of the antibody with the target proteins
  • Efficient and easy coupling
  • Antibodies are covalently coupled to Dynabeads M-270 Epoxy beads, minimizing the risk of bound antibody contaminating your final eluate
  • Exhibit ultra-low background binding and don't require blocking before use.
  • Binding, washing, and elution buffers are optimized for consistent and reliable results that enable purification of complex protein structures of any size
  • Components are analytical grade and compatible with protease and phosphatase inhibitors

  • Protocol describes coupling, sample preparation (detergent and cryolysis methods), co-IP and elution, and is compatible with western blot/silver stain, Coomassie™; stain, mass spectrometry, or extraction of nucleic acids from isolated DNA/RNA binding protein complexes
  • Easy handling, easily separated, washed, and eluted using a DynaMag™ magnet, and the magnetic separation properties of the Dynabeads

Immunoprecipitation, Protein and Peptide Purification, Protein Complex Pull-Down, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room Temperature

Spécification

Spécification

Manual
Freeze Dried
40 reactions
Vidéos
Documents
Promotions

Promotions

  • Economisez jusqu'à 61% pour une durée limitée seulement
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    2019-12-31

    La remise sera appliquée aux commandes éligibles reçues par Fisher Scientific entre 01/09/19 et 31/12/19. Le code promotionnel doit être renseigné lors de la commande. La remise s'applique sur le prix affiché en vigueur au moment où la commande est reçue. La promotion ne peut être cumulée avec d'autres remises ou promotions. L'offre est nulle et non avenue si interdite, assujettie à des exigences de licence ou restreinte par des lois ou réglementations fédérales, nationales, régionales ou locales, ou des politiques institutionnelles et gouvernementales. D'autres restrictions peuvent s'appliquer.

    Termes et Conditions

For Research Use Only. Not for use in diagnostic procedures.