Thermo Scientific™ POROS™ CaptureSelect™ CH1-XL Affinity HPLC Columns

CaptureSelect CH1-XL Affinity HPLC columns recognize the CH1 domain of human IgG antibodies, enabling quantitation and small-scale purification of all IgG subclasses (IgG1, IgG2, IgG3, and IgG4), as well as Fab fragments and fusion proteins containing the intact CH1 domain, independent of light-chain iso-type and source material.Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle.

Features of POROS CaptureSelect CH1-XL Affinity HPLC columns include:

• Versatile—applicable for IgG Fab fragments, all human IgG subclasses, and IgG fusion proteins with intact CH1 domain
• Specific—no cross-binding to bovine IgG
• Fast—POROS resins enable quantitation or purification in minutes
• Hands-off operation—HPLC method can be automated for a streamlined operation
• Robust—designed to deliver high-performance purification over hundreds of chromatography cycles

High speed with high specificity

POROS high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 μm filtration) is required, and the eluted product is highly purified and suitable for further analysis, including mass spectrometry.

Automation

POROS Affinity HPLC columns are for use with HPLC systems, which allows the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligand enables one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple cycles

Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regimen results in strong re-use performance. POROS Affinity HPLC columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

Main characteristics

• Protein binding capacity: 26.2 mg/mL for human IgG; 8.8 mg/mL human Fab fragment
• Species specificity: human
• Column material: PEEK polymer
• Frit: 2 μm, titanium
• POROS resin: cross-linked poly(styrene-divinylbenzene)
• CaptureSelect ligands: single-domain monospecific antibody fragment produced in Saccharomyces cerevisiae