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Applied Biosystems™ resDNASEQ™ Quantitative MDCK DNA Kits
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Comprend:
Assay Kit and Sample Preparation
Assay Kit only
Conditionnement:
100 réactions
Description
The resDNASEQ™ Quantitative MDCK DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ™ system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of MDCK cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types, from in-process samples to final product, if the sample contains high molecular weight or sheared DNA.
Features of the resDNASEQ Quantitative MDCK DNA Kit include:
• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan™ primer/probe mix, and MDCK genomic DNA standard
This kit is available with or without sample preparation reagents in the form of a PrepSEQ™ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.
The resDNASEQ™ Quantitative MDCK DNA Kit is designed to provide highly sensitive detection of MDCK DNA, allowing you to use small sample volumes to generate accurate results. The broad linear range of TaqMan™ real-time qPCR technology allows you to test samples with variable levels of MDCK DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.
Easy to use
With the resDNASEQ™ Quantitative MDCK DNA Kit you perform qPCR analysis to compare the amount of MDCK DNA in your test samples to a standard curve generated with known amounts of purified MDCK genomic DNA standard. The resDNASEQ™ kit includes almost everything you need to carry out these steps, from master mix to TaqMan™ primer/probe mix to MDCK genomic DNA standard, as well as step-by-step instructions.
Specific to MDCK DNA, no cross-reactivity with unrelated DNA
The target of the assay is highly specific—it only detects a MDCK-specific region of a multicopy genetic element. We selected this region using extensive bioinformatic analysis of multiple related and unrelated species.
Consistent performance even with fragmented DNA
For accurate quantitation of residual MDCK DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ™ system you get consistent assay performance across a range of DNA fragment sizes.
• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan™ primer/probe mix, and MDCK genomic DNA standard
This kit is available with or without sample preparation reagents in the form of a PrepSEQ™ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.
The resDNASEQ™ Quantitative MDCK DNA Kit is designed to provide highly sensitive detection of MDCK DNA, allowing you to use small sample volumes to generate accurate results. The broad linear range of TaqMan™ real-time qPCR technology allows you to test samples with variable levels of MDCK DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.
Easy to use
With the resDNASEQ™ Quantitative MDCK DNA Kit you perform qPCR analysis to compare the amount of MDCK DNA in your test samples to a standard curve generated with known amounts of purified MDCK genomic DNA standard. The resDNASEQ™ kit includes almost everything you need to carry out these steps, from master mix to TaqMan™ primer/probe mix to MDCK genomic DNA standard, as well as step-by-step instructions.
Specific to MDCK DNA, no cross-reactivity with unrelated DNA
The target of the assay is highly specific—it only detects a MDCK-specific region of a multicopy genetic element. We selected this region using extensive bioinformatic analysis of multiple related and unrelated species.
Consistent performance even with fragmented DNA
For accurate quantitation of residual MDCK DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ™ system you get consistent assay performance across a range of DNA fragment sizes.
Spécification
Spécification
| Contenu et stockage | resDNASEQ MDCK DNA Control • MDCK DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C • DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use resDNASEQ MDCK DNA Real-Time PCR Reagents • Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light • MDCK DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light • Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use PrepSEQ Nucleic Acid Extraction Kit • Lysis Buffer (2 bottles, 50 mL/bottle) • Binding Solution/Isopropanol (1 empty bottle) • Wash Buffer Concentrate (2 bottles, 26 mL/bottle) • Elution Buffer (1 bottle, 25 mL) • Proteinase K Buffer (1 bottle, 50 mL) • Magnetic Particles (2 tubes, 1.5 mL/tube) • Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C • PK Buffer II (1 bottle, 11 mL) Store all at room temperature unless otherwise indicated. PrepSEQ Residual DNA Sample Preparation Kit • Proteinase K (20 mg/mL), 1 tube, 1.25 mL • Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL • Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tube Store all at or below –20°C. |
| Méthode de détection | Amorce-sonde |
| Format | Kit |
| Méthode PCR | qPCR |
| Cellule hôte | MDCK |
| Marqueur ou colorant | FAM |
| Gamme de produits | resDNASEQ |
| Quantité | 100 reactions |
| Nbre de réactions | 100 réactions |
| Comprend | Assay Kit and Sample Preparation |
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