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Invitrogen™ Human ZAP-70, His Tag Recombinant Protein
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Quantité:
20 μg
Conditionnement:
20µg
Description
Protein Form: Full Length, Recombinant, Cytoplasmic Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical europium phospho-specific activity, Biochemical binding/kinetic System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Z'-LYTE™, Omnia™ Bulk Substrate.
ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.
Spécification
Spécification
| Numéro d’adhésion | P43403 |
| Concentration | See Label |
| À utiliser avec (application) | Kinase Assay |
| Formule | 25mM HEPES with 0.5mM DTT, 50% glycerol, 150mM NaCl and no preservative; pH 7.5 |
| Identification génétique (Entrez) | 7535 |
| Poids moléculaire | 70.1 kDa |
| Nom | Human ZAP-70, His Tag |
| Plage de pH | 7.5 |
| Méthode de purification | Purified |
| Quantité | 20 μg |
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