Invitrogen™ CD120a (TNF Receptor I) Monoclonal Antibody (55R-170), Functional Grade, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

The 55R-170 MAb reacts with mouse Tumor Necrosis Factor Receptor I (TNFRI, TNFR-p55, TNFRSF1A, CD120a). TNFRI is expressed in a variety of cell types. The soluble TNF receptors are truncated forms of cell surface receptors with neutralizing activity on both TNF-alpha and TNF-beta. It has been suggested that the proinflammatory and immunosuppressive properties of TNF segregate at the level of its receptors and that the pool of TNF bound to soluble receptors could represent a reservoir to control the TNF release. Applications Reported: The 55R-170 antibody has been reported for use in ELISA, neutralization of mouse TNFR I bioactivity and flow cytometric analysis. Applications Tested: The Functional Grade Purified 55R-170 antibody has been tested by LAL assay to verify low endotoxin levels and in bioassay for neutralization of mouse TNFR I bioactivity. The 55R-170 antibody at 25 μg/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse TNF alpha in an assay of TNF alpha induced cytotoxicity of L929 cells. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the Best Protocols section. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.