CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Description: The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages. Applications Reported: This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This eBio84-3C1 (84-3C1) antibody has been tested by flow cytometric analysis of human peripheral blood cells and by immunohistochemistry of formalin-fixed paraffin embedded human spleen using low pH antigen retreival at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.