Invitrogen™ CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Description: The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71. Applications Reported: This OKT9 (OKT-9) antibody has been reported for use in flow cytometric analysis. Applications Tested: This OKT9 (OKT-9) antibody has been tested by flow cytometric analysis of PHA-stimulated human peripheral blood mononuclear cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.