Invitrogen™ COX2 Monoclonal Antibody (COX 229), eFluor™ 450, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Description: This COX 229 monoclonal antibody recognizes human COX2 also known as PTGS2. This antibody has been reported to cross-react with the mouse and rat COX2. For optimal performance we recommend using this antibody with the Foxp3 / Transcription Factor Staining Buffer Set and protocol. This antibody is predicted to cross-react with the mouse and rat homologue. Applications Reported: This COX 229 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This COX 229 antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product No. 00-5523-00) and protocol. Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins located at www.thermofisher.com/flowprotocols. This may be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor 450 is an alternative to Pacific Blue.