Invitrogen™ F(ab')2-Goat anti-Human IgG, IgM (H+L) Secondary Antibody, Functional Grade, eBioscience™

Description

Description: This polyclonal F(ab')2 fragment recognizes human IgM and IgG. Reactivity to bovine has been removed. This polyclonal antibody can be used to crosslink and activate B lymphocytes. Applications Reported: This polyclonal F(ab')2 Goat Anti-Human IgM+IgG Functinoal Grade Purified has been reported for use in functional assays (such as B cell activation). Concentrations should be determined empirically for optimal performance in the assay of interest. Applications Tested: F(ab')2 Anti-Human IgM+IgG Functional Grade Purified has been tested by in vitro stimulation of normal human peripheral blood cells. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.