Invitrogen™ Goat anti-Rabbit IgG (H+L) Secondary Antibody, AP

Description

This Alkaline Phosphatase Conjugated secondary antibody can be used with the ELISA-Light Immunoassay, Western-Light, and Western-Star Western Blotting Detection Systems. This labeled secondary antibody ensures minimal nonspecific cross-reactivity. Life Technology has optimized its alkaline phosphatase secondary antibody conjugates for Western blotting or immunoassay procedures incorporating chemiluminescent 1,2-dioxetane enzyme substrates. Optimized for use in blotting applications with dioxetane-based substrate solutions (CSPD or CDP-Star substrates), providing consistent experiment-to-experiment results. High specific activity of conjugates provides high sensitivity of detection with dioxetane-based substrate solutions (CSPD or CDP-Star substrates). Low non-specific binding provides highest possible signal-to-noise in western blotting and immunoassay applications. Easy to Use The recommended initial working dilution for the labeled antibodies is 1:5,000 for AP conjugates. The conjugate is tested and optimized for low background with chemiluminescent detection procedures.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.