Invitrogen™ High Endothelial Venule Marker Monoclonal Antibody (MECA-79), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

Description: This MECA-79 monoclonal antibody recognizes a sulfated carbohydrate epitope present on human and mouse high endothelial venules (HEVs) called peripheral lymph node addressin (PNAd). This glycoprotein is expressed on the luminal surface and in the cytoplasm of HEVs in lymphoid tissues, including peripheral and mesenteric lymph nodes and, to a lesser extent, Peyer's patches. The MECA-79 antigen has also been detected on follicular B cells in the spleen and appendix in humans, as well as at sites of chronic inflammation. Post-capillary venules or large vessels in the spleen, thymus, or non-lymphoid tissues do not express the MECA-79 antigen and thus cannot be detected with this antibody. This addressin is involved in lymphocyte infiltration and adhesion into secondary lymphoid tissues. The MECA-79 antibody has been reported to inhibit CD62L (L-selectin)-dependent lymphocyte emigration to HEVs and adhesion in lymph nodes and tonsils. Applications Reported: This MECA-79 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections (IHC-P). Applications Tested: This MECA-79 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human tonsil with low pH antigen retrieval at less than or equal to 20 μg/mL. It is recommended that the anitbody be titrated for optimal performance in the assay of interest.

HEV peripheral lymph node addressin (PNAd) is a glycoprotein is expressed on the luminal surface and in the cytoplasm of HEVs in lymphoid tissues, including peripheral and mesenteric lymph nodes and, to a lesser extent, Peyer's patches. The MECA-79 antigen has also been detected on follicular B cells in the spleen and appendix in humans, as well as at sites of chronic inflammation. Post-capillary venules or large vessels in the spleen, thymus, or non-lymphoid tissues do not express the MECA-79 antigen and thus cannot be detected with this antibody. This addressin is involved in lymphocyte infiltration and adhesion into secondary lymphoid tissues.