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  6. Invitrogen™ ID2 Monoclonal Antibody (ILCID2), APC, eBioscience™, Invitrogen™

Invitrogen™ ID2 Monoclonal Antibody (ILCID2), APC, eBioscience™, Invitrogen™

Code produit 15943224
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Quantité:
100 μg
25 μg
Conditionnement:
100µg
25µg
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15943224 25 μg 25µg
15953224 100 μg 100µg
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Code produit 15943224 Fournisseur Invitrogen™ Code fournisseur 17947580

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Description

Mouse Monoclonal Antibody

Description: This ILCID2 monoclonal antibody recognizes mouse and human ID2. This ILCID2 antibody requires Foxp3/Transcription Factor Staining Buffer set and will perform poorly when used with standard IC Fixation & Permeabilization Buffer Set and protocol. Applications Reported: This ILCID2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This ILCID2 antibody has been tested by flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 1.0 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

The inhibitor of DNA binding 2 (ID2) protein is essential for promoting the development of a number of cell types including natural killer cell, erythroid precursors, as well as, CD8+ and CD4+ T-cell. A lack of ID2 promotes B-cell development. The chief role of this protein is to associate with ubiquitously-expressed E proteins, and other proteins of the basic helix-loop-helix (HLH) family, preventing them from binding to DNA and activating transcription. Activation of ID2 is critical for the development of the natural killer cell lineage.
TRUSTED_SUSTAINABILITY

Spécification

Antigène ID2
Applications Flow Cytometry
Classification Monoclonal
Clone ILCID2
Concentration 0.2 mg/mL
Conjugué APC
Formule PBS with 0.09% sodium azide; pH 7.2
Expression ID2
Numéro d’ordre du gène P41136, Q02363
Alias de gène Ac2-300; AI255428; bHLHb26; C78922; cell growth-inhibiting gene 8; class B basic helix-loop-helix protein 26; DNA-binding protein inhibitor ID2; DNA-binding protein inhibitor ID-2; GIG8; helix-loop-helix protein ID2; Id2; Id-2; ID2A; ID2H; Idb2; Inhibitor of differentiation 2; inhibitor of DNA binding 2; inhibitor of DNA binding 2, dominant negative helix-loop-helix protein; inhibitor of DNA binding 2, HLH protein; MGC26389; OTTHUMP00000140258; OTTHUMP00000200297
Symboles de gène(s) ID2
Espèces hôtes Mouse
Immunogène Purified recombinant fragment of human ID2, corresponding to amino acids 1-134 expressed in E. coli.
Méthode de purification Affinity chromatography
Quantité 25 μg
État réglementaire RUO
Primaire ou secondaire Primary
Identification génétique (Entrez) 15902, 3398
Espèces cibles Human, Mouse
Contenu et stockage 4°C, store in dark, DO NOT FREEZE!
Type de produit Antibody
Forme Liquid
Isotype IgG1 κ
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