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EnquireBio SingleStep Poly-HRP anti-Rabbit IgG (H+L) Secondary Antibody
Goat Polyclonal Antibody
Marque: EnquireBio Q2AB2-500ug
Informations supplémentaires : Poids : 0.01000kg
DescriptionSingleStep™ Poly-HRP (Horse Radish Peroxidase) conjugated goat anti-rabbit secondary antibodies provide signal amplification over traditional HRP conjugated reagents in immunohistochemistry (IHC) staining using primary antibodies from a rabbit host. The signal amplification afforded by these HRP polymer conjugated antibodies is sufficient to enable application of a single secondary antibody unlike standard HRP conjugated reagents which utilize an unconjugated or biotinylated secondary antibody and then an additional HRP conjugated secondary antibody or HRP conjugted streptavidin to visualize staining. These additional staining steps increase background staining and variability between samples which is obviously undesirable in IHC. SingleStep™ goat anti-rabbit poly-HRP secondary antibodies are sourced from the industry leading secondary antibody manufacturer and are then conjugated to a carefully controlled number of HRP molecules to facilitate excellent tissue penetration without sacrificing signal amplification. Our predilute products also incorporate our enhanced IHC staining buffer which contains optimized stabilizers and blocking reagents to ensure maximum sensitivity and high reproducibility in IHC in most sample types.
|Pre-dilute antibody is in proprietary signal enhancement buffer composed of protein based blocking agent (non-BSA), non-ionic detergent, preservative (non-azide), and HRP-stabilizer at pH 7.4. Concentrated format also contains 50% glycerol.|
|Generation of this anti-Poly-HRP Anti-Rabbit IgG (H+L) Secondary Antibody antibody was achieved by immunizing a Goat host with Pooled population of Rabbit IgG|
|Store at 4°C. Do not freeze. Stable for >1 year if stored properly.|
|Antibody is initially purified by ammonium sulfate precipitation followed by cross-adsorption to ensure minimal cross-reactivity to human, bovine, and equine antibodies. Purification of SingleStep(TM) poly-HRP conjugated goat anti-rabbit secondary is achieved via size exclusion liquid chromatography.|