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Invitrogen™ V5 Tag Monoclonal Antibody (TCM5), eBioscience™, Invitrogen™

Code produit 15287947
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25 μg
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25µg
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15287947 25 μg 25µg
15297947 100 μg 100µg
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Code produit 15287947 Fournisseur Invitrogen™ Code fournisseur 14679680

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Mouse Monoclonal Antibody

Description: This TCM5 monoclonal antibody recognizes recombinant proteins containing a V5 epitope tag. The V5 epitope is naturally present on the P and V proteins of the paramyxovirus, SV5. TCM5 specifically recognizes the sequence GKPIPNPLLGLDST. Applications Reported: This TCM5 antibody has been reported for use in western blotting, and immunocytochemistry. Applications Tested: This TCM5 antibody has been tested by western blot and immunofluorescent staining of V5-transfected cells and can be used at less than or equal to 1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
TRUSTED_SUSTAINABILITY

Spécification

Antigène V5 Tag
Applications Western Blot, Immunocytochemistry
Classification Monoclonal
Clone TCM5
Concentration 0.5 mg/mL
Conjugué Unconjugated
Formule PBS with 0.09% sodium azide; pH 7.2
Alias de gène simian virus 5 antibody; SPV5gp2; sv5; V5; v-5; V5 Epitope Tag
Espèces hôtes Mouse
Méthode de purification Affinity chromatography
Quantité 25 μg
État réglementaire RUO
Primaire ou secondaire Primary
Espèces cibles Tag
Contenu et stockage 4°C
Type de produit Antibody
Forme Liquid
Isotype IgG2b κ
Molécule cible Tag
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For Research Use Only.

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