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6x-His Tag Monoclonal Antibody (AD1.1.10), HRP, Invitrogen™

Mouse Monoclonal Antibody

Marque:  Invitrogen MA180218

Code nomenclature Nacres: NA.41

Informations supplémentaires : Poids : 0.10000kg

Code produit. 10603374

  • 590.00€ / 100µg
Expédition estimée: 01-07-2024
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Description

Description

Mouse anti Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags.

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Spécification

Spécification

6x-His Tag
Monoclonal
1 mg/mL
PBS with 0.01% thimerosal; pH 7.4
Mouse
Protein G
RUO
Tag
Antibody
IgG1
ELISA, Western Blot
AD1.1.10
HRP
6His tag; 6X His; His Tag; Histidine Tag
PAX6 transcription factor linked to histidine tag.
100 μg
Primary
4° C, do not freeze
Liquid
Tag
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