PeproTech APRIL Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: AVLTQKQKKQ HSVLHLVPIN ATSKDDSDVT EVMWQPALRR GRGLQAQGYG VRIQDAGVYL LYSQVLFQDV TFTMGQVVSR EGQGRQETLF RCIRSMPSHP DRAYNSCYSA GVFHLHQGDI LSVIIPRARA KLNLSPHGTF LGFVKL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human APRIL. Anti-Human APRIL-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hAPRIL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human APRIL (500-P192) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hAPRIL. Western Blot: To detect Human APRIL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human APRIL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P192BT-1MG will be provided as 2 x 500 μg

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for A PRoliferation-Inducing Ligand), TALL2 (for TNF and ApoL-related Leukocyte-expressed Ligand 2), and TRDL 1a (for TNF related death ligand 1a) in human and mouse. Two receptors for APRIL were recently identified and designated TACI and BCMA. APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kB, and induces cell death. APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer.