Invitrogen™ BCL6 Monoclonal Antibody (BCL-DWN), APC, eBioscience™, Invitrogen™

Description

Description: This BCL-DWN monoclonal antibody reacts with mouse Bcl-6, which is also known as B-cell lymphoma 6. A member of the POZ family of transcriptional repressors, Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79-kDa protein is essential for germinal center B cell proliferation and survival. Moreover, during B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt's lymphoma, and non-Hodgkin's lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1. Applications Reported: This BCL-DWN antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This BCL-DWN antibody has been tested by intracellular staining followed by flow cytometric analysis of splenocytes isolated from sheep red blood cell-immunized mice using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.03 μg per test.

BCL6 Monoclonal antibody specifically detects BCL6 in Mouse samples. It is validated for Flow Cytometry.