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Invitrogen™ BrdU Polyclonal Antibody
Rabbit Polyclonal Antibody
Brand: Invitrogen™ PA532256
This item is not returnable.
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Description
Recommended positive controls: HeLa cells were treated with 100uM BrdU for 4hrs, U343 cells were treated with 10 μM BrdU for 18hrs. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
Specifications
| BrdU | |
| Polyclonal | |
| Unconjugated | |
| 5-Bromo-2-deoxyuridine; Bromodeoxyuridine | |
| BrdU. | |
| 100 μL | |
| Primary | |
| Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
| Liquid | |
| Chemical |
| Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry | |
| 1 mg/mL | |
| PBS with 20% glycerol and 0.025% ProClin 300; pH 7 | |
| Rabbit | |
| Antigen affinity chromatography | |
| RUO | |
| Chemical | |
| Antibody | |
| IgG |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- ALG9 ANTIBODY
Signal Word
- Attention
Hazard Category
- Sensibilisation cutanée Catégorie 1
Hazard Statement
- H317-Peut provoquer une allergie cutanée.
Precautionary Statement
- P261-Éviter de respirer les poussières/fumées/gaz/brouillards/vapeurs/ aérosols.
- P272-Les vêtements de travail contaminés ne devraient pas sortir du lieu de travail.
- P280-Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/ du visage.
- P302+P352-EN CAS DE CONTACT AVEC LA PEAU: Laver abondamment à l'eau/.
- P333+P313-En cas d'irritation ou d'éruption cutanée: consulter un médecin.
- P362+P364-Enlever les vêtements contaminés et les laver avant réutilisation.
- P501b-Éliminer le contenu/récipient conformément à la réglementation locale/régionale/nationale/internationale.
Supplemental information
- MIXTURE LIST-Contient: Mixture, 3(2H)-isothiazolone, 5-chloro-2-methyl-2-methyl-3(2H)-isothiazolone
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