PeproTech CCL1 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human I-309 (CCL1). Anti-Human I-309 (CCL1)-specific antibody was purified by affinity chromatography employing an immobilized Human I-309 (CCL1) matrix. Sandwich ELISA: To detect Human I-309 (CCL1) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human I-309 (CCL1) (500-P110Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human I-309 (CCL1). Western Blot: To detect Human I-309 (CCL1) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human I-309 (CCL1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.