PeproTech CCL5 (RANTES) Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine RANTES (CCL5). Anti-Murine RANTES (CCL5)-specific antibody was purified by affinity chromatography employing an immobilized Murine RANTES (CCL5) matrix. Sandwich ELISA: To detect Murine RANTES (CCL5) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine RANTES (CCL5) (500-P118Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine RANTES (CCL5). Western Blot: To detect Murine RANTES (CCL5) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine RANTES (CCL5) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.