Invitrogen™ CD13 Monoclonal Antibody (WM-15 (WM15)), eBioscience™, Invitrogen™

Description

Description: The WM-15 monoclonal antibody reacts with human CD13, also known as aminopeptidase N. CD13 is a transmembrane ectoenzyme occurring on monocytes and granulocytes but not lymphocytes in blood. It is also reportedly on a wide variety of cells and in a wide variety of tissues, including endothelial cells, epithelial cells, fibroblasts, kidney proximal tubules, intestine, and placenta. CD13 is reported to have many different functions including degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and adhesion and migration of cells. Applications Reported: The WM-15 (WM15) antibody) has been reported for use in flow cytometric analysis, and microscopy. Applications Tested: The WM-15 (WM15) antibody has been tested by flow cytometric analysis of lysed whole blood. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD13 (ANPEP) is a 150-170 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. CD13 is a metalloprotease that preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 also has a role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is expressed by granulocytes, monocytes, myeloid leukemia cells, and is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas. CD13 functions as an aminopeptidase enzyme and is also alpha receptor for coronavirus.