Invitrogen™ CD185 (CXCR5) Monoclonal Antibody (SPRCL5), APC, eBioscience™, Invitrogen™

Beschreibung

Description: This SPRCL5 monoclonal antibody reacts with Mouse CD185. CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt's lymphoma. In peripheral blood, CXCR5 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), as well as on a subpopulation of memory (CD45RO+) T cells. CXCR5+ circulating T cells are in a resting state and migrate to the lymph nodes due to expression of CCR7 and CD62L. In tonsil, CXCR5 is expressed on nearly all CD4+ cells along with CD45RO and such activation markers as CD69 and ICOS. Tonsillar CXCR5+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing B cell help. Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular T cell migration into the B cell follicles of germinal centers in response to CXCL13, making CXCR5 an established marker of follicular helper T cells. This SPRCL5 does not see a formaldehyde fixed epitope, therefore staining should be done prior to fixation. Applications Reported: This SPRCL5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SPRCL5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test.

CD185 (CXCR5) Monoclonal antibody specifically detects CD185 (CXCR5) in Mouse samples. It is validated for Flow Cytometry.