CD205 (DEC-205) Rat anti-Mouse, Brilliant Violet 421, Clone: V18-949, BD

Description

The V18-949 monoclonal antibody specifically recognizes CD205 which is also known as Dendritic and thymic Epithelial Cells, 205 kDa (DEC-205). CD205 (DEC-205) is a type 1 transmembrane glycoprotein that is encoded by Ly75 (lymphocyte antigen 75). CD205 (DEC-205) is highly expressed by both CD8+ and CD103+ dendritic cell subsets and Langerhans cells. It is variably expressed by some other dendritic cell types, Gr1+ (Ly-6G and Ly-6C) bone marrow cells, B cells, macrophages, granulocytes, and mucosal and thymic cortical epithelial cells. CD205 (DEC-205) belongs to the C-type lectin receptor family and functions as a pattern recognition receptor and an endocytic receptor that participates in the cellular processing and presentation of foreign or self antigens to initiate immunity or tolerance.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.