PeproTech CD25 (soluble) Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQ. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-2 Receptor alpha. Anti-Human sIL-2 Receptor alpha-specific antibody was purified by affinity chromatography employing an immobilized Human sIL-2 Receptor alpha matrix. Sandwich ELISA: To detect Human sIL-2 Receptor alpha by sandwich ELISA (using 100 μL/well) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sIL-2 Receptor alpha (500-P22RBT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human sIL-2 Receptor alpha. Western Blot: To detect Human sIL-2 Receptor alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sIL-2 Receptor alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.