Invitrogen™ CD49a (Integrin alpha 1) Monoclonal Antibody (TS2/7), PE, eBioscience™, Invitrogen™

Description

Description: The monoclonal antibody TS2/7 reacts with human CD49a, also known as integrin alpha 1. CD49a is always associated with CD29 (integrin beta 1) to form a type I transmembrane protein called very late antigen-1 (VLA1). This heterodimer is expressed by endothelial cells, activated T cells, monocytes, a subset of NK cells, a subset of memory T cells, smooth muscle cells, and mesenchymal stem cells. CD49a binds to collagen IV strongly, but also binds to collagen I, laminin, and semaphorin7A. CD49a is involved in cell survival, proliferation, homing, and inflammatory responses. Applications Reported: This TS2/7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TS2/7 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.