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CD56 (NCAM) Mouse anti-Human, Non-human primate, Rhesus Monkey, Super Bright 645, Clone: TULY56, eBioscience™

Mouse Monoclonal Antibody

Marque:  Invitrogen 64-0566-42

Informations supplémentaires : Poids : 0.01000kg

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Code produit. 15875663

  • 372.00€ / 100 pièces
Date d’expédition estimée: 22-12-2021

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Description

Description

Description: This OV9A2 monoclonal antibody reacts with human CD59, which is also known as Protectin. This GPI-linked membrane glycoprotein shares structural homology with the murine Ly-6 superfamily. CD59 is expressed on all human lymphocytes, monocytes, granulocytes, and erythrocytes. This protein is also expressed on non-hematopoietic cells, including endothelial cells and neurons. By binding the complement components C8 and C9, CD59 inhibits assembly of the membrane attack complex and cytolytic activity by complement. CD59 interacts with CD2 to modulate T cell adhesion, and also plays a role in T cell activation. Finally, altered expression of CD55 and CD59 on human peripheral blood cells has been observed in patients with systemic lupus erythematosus (SLE). Crossblocking studies indicate that the OV9A2 monoclonal antibody binds the same epitope as MEM-43. Applications Reported: This OV9A2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This OV9A2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins.
Spécification

Spécification

CD56 (NCAM)
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P13591
NCAM1
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
4684
Flow Cytometry
TULY56
Super Bright 645
NCAM1
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Non-human Primate, Rhesus Monkey
Vidéos
FDS
Documents
Certificats
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For Research Use Only