PeproTech CLEC11A Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human SCGF-beta. Anti-Human SCGF-beta-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hSCGF-beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SCGF-beta (500-P99G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hSCGF-beta. Western Blot: To detect hSCGF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hSCGF-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P99GBT-1MG will be provided as 2 x 500 μg

This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined.