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Cytokeratin, pan Antibody (AE-1/AE-3) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 4 publications

Marque:  Novus Biologicals NBP2-33200

Code nomenclature Nacres: NA.46

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Code produit. 18715743

  • 670.00€ / 0.10mg

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Description

Description

Cytokeratin, pan Monoclonal specifically detects Cytokeratin, pan in Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit, Reptile, Zebrafish samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), CyTOF-ready, Single Cell Western, Dual RNAscope ISH-IHC.
TRUSTED_SUSTAINABILITY
Spécification

Spécification

pan Cytokeratin
Monoclonal
1.0 mg/mL
Western Blot 0.5-1ug/ml, Flow Cytometry 0.1 - 1 ug/million cells, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml, Flow (Intracellular), CyTOF-ready, Dual RNAscope ISH-IHC
Mouse
Protein A or G purified
RUO
Primary
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
IgG1 κ
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), CyTOF
AE-1/AE-3
Unconjugated
KRT1
This Cytokeratin, pan Antibody (AE-1/AE-3) was developed against total keratin isolated from human epidermal callus was used as immunogen to generate the pan cytokeratin antibodies AE1 + AE3 (Woodcock-Mitchell, 1982).
0.1 mg
Apoptosis, Cancer, Cell Biology, Cellular Markers, Signal Transduction
3848
Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit, Reptile, Zebrafish
Purified
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