IGFBP-1 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-BP1. Anti-Human IGF-BP1-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hIGF-BP1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IGF-BP1 (500-P228) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIGF-BP1. Western Blot: To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIGF-BP1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P228BT-1MG will be provided as 2 x 500 μg

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.