PeproTech IL-12 p70 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: p35 subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS . Preparation: Produced from sera of goats immunized with highly pure Rec. Hu IL-12. Anti-Human IL-12-specific antibody was purified by affinity chromatography employing an immobilized Human IL-12 matrix. Sandwich ELISA: To detect Human IL-12 by sandwich ELISA (using 100 uL/well antibody solution) a concentration of 0.5-2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-12 (500-P154GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-12. Western Blot: To detect Human IL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.