MIP-1 gamma Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MIP-1gamma (CCL9/10). Anti-Murine MIP-1gamma (CCL9/10)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Murine MIP-1gamma (CCL9/10) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MIP-1gamma (CCL9/10) (500-P117) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MIP-1gamma (CCL9/10). Western Blot: To detect Murine MIP-1gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine MIP-1gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P117BT-1MG will be provided as 2 x 500 μg

MIP-1gamma is a CC chemokine found in murine blood and a wide variety of murine tissues, with no known human homolog. MIP-1gamma signals through the CCR1 receptor. MIP-1gamma chemoattracts neutrophils, and also inhibits colony formation of bone marrow myeloid immature progenitors. MIP-1gamma contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines.