missing translation for 'onlineSavingsMsg'
Learn More

CD19 Mouse anti-Human, BUV563, Clone: SJ25C1, BD

Mouse Monoclonal Antibody

Marque:  BD Biosciences 565697

Informations supplémentaires : Poids : 0.00800kg

 Afficher plus de versions de ce produit

Code produit. 15874469

  • 575.00€ / 1 pièce
Date d’expédition estimée: 17-12-2021

Veuillez vous pour pouvoir commander cet article. Besoin d'un compte web? Créer le vôtre dès maintenant!


Description

Description

The SJ25C1 monoclonal antibody specifically binds to CD19, a B lymphocyte-lineage differentiation antigen. CD19, a 90-kDa transmembrance glycoprotein, is a member of the immunoglobulin superfamily and is expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. Terminally differentiated plasma cells do not express CD19. On the surface of mature B cells, the CD19 molecule associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to promote cellular activation. Studies with CD19-deficient mice have suggested that the level of CD19 expression affects the generation and maturation of B cells in the bone marrow and periphery. B-1 lineage B cells, also known as CD5+ B cells, are drastically reduced or absent in CD19-deficient mice. Increased levels of CD19 expression correlate with increased frequencies of peritonal and splenic B-1 cells and reduced numbers of conventional B lymphocytes in the periphery. CD19 participates in B-lymphocyte development, B-cell activation, maturation of memory B cells and regulation of tolerance. CD19 has also been detected on peritoneal mast cells, co-localized with CD21/CD35, and it is proposed to play a role in complement-mediated mast-cell activation.

The antibody was conjugated to BD Horizon BUV563 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 which has an Ex Max of 348nm and an acceptor dye. The tandem has an Em Max at 563 nm. BD Horizon BUV563 can be excited by the 355nm ultraviolet laser. On instruments with a 561nm Yellow-Green laser, the recommended bandpass filter is 585/15nm with a 535nm long pass to minimize laser light leakage. When BD Horizon BUV563 is used with an instrument that does not have a 561nm laser, a 560/40nm filter with a 535nm long pass may be more optimal. Due to the excitation and emission characteristics of the acceptor dye, there may be spillover into the PE and PE-CF594 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Spécification

Spécification

CD19
SJ25C1
B4; B-lymphocyte antigen CD19; Leu-12
Affinity Purified
Human NALM1 + NALM16 cells
Affinity Chromatography
RUO
Primary
Human
Flow Cytometry
Brilliant Ultraviolet 563
Aqueous buffered solution containing ≤0.09% sodium azide.
Mouse
IgG1, κ
100 Tests
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Vidéos
FDS
Documents
Certificats
Promotions

Promotions

For Research Use Only.