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MRCL3, p Ser19, p Ser20 Antibody, Novus Biologicals™
Rabbit Polyclonal Antibody
Marque: Novus Biologicals NB100-1687
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Description
MRCL3 Polyclonal specifically detects MRCL3 in Human, Mouse samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin.
Spécification
| MRCL3 (p Ser19, p Ser20) | |
| Polyclonal | |
| Unconjugated | |
| 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide | |
| MLCBMyosin RLC, MRCL3, MRLC3MLC-2B, MYL2B, myosin regulatory light chain 12A, Myosin regulatory light chain 2, nonsarcomeric, myosin regulatory light chain 3, myosin regulatory light chain MRCL3, Myosin regulatory light chain MRLC3, myosin, light chain 12A, regulatory, non-sarcomeric, myosin, light polypeptide, regulatory, non-sarcomeric (20kD), RLC | |
| Rabbit | |
| 19.79 kDa | |
| 0.05 mg | |
| Primary | |
| This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The antibody is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phosphospecific polyclonal antibody is specific for the phosphorylated pS19/pS20 form of the protein, depending on the source origin of the protein. Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA. Cross reactivity is expected with myosin light chain from human and mouse. Reactivity with the protein from other species has not been determined. However, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected. | |
| Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles. |
| Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin) | |
| 1 mg/ml | |
| Western Blot 1:500-1:2000, ELISA 1:10000-1:30000, Immunohistochemistry 2.5 ug/ml, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:10-1:500 | |
| P19105 | |
| MYL12A | |
| Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH). (Uniprot: P19105) | |
| Affinity Purified | |
| RUO | |
| 10627 | |
| Human, Mouse | |
| IgG |
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Nom du produit
For Research Use Only
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