Invitrogen™ NKG2A/C/E Monoclonal Antibody (20d5), Functional Grade, eBioscience™, Invitrogen™

Description

Description: The 20d5 monoclonal antibody reacts with the mouse NKG2A, C, and E. NKG2 molecules belong to a C-type lectin-like family of cell surface receptors expressed by mouse NK and NKT cell lineages. NKG2 molecules form heterodimeric complexes with CD94 and are responsible for recognition of non-classical MHC class I antigen Qa-1. When co-staining with NKG2A^B6 clone 16a11, it is important to stain for NKG2A-B6 first then subsequently with NKG2A/C/E, as steric hindrance has been observed if co-stained concurrently. Applications Reported: This 20d5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 20d5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. The protein encoded by this gene belongs to the killer cell lectin-like receptor family, also called NKG2 family, which is a group of transmembrane proteins preferentially expressed in NK cells. This family of proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain. This protein forms a complex with another family member, KLRD1/CD94, and has been implicated in the recognition of the MHC class I HLA-E molecules in NK cells. The genes of NKG2 family members form a killer cell lectin-like receptor gene cluster on chromosome 12. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed.