PDIR Polyclonal Antibody, Invitrogen™

Description

PA3-007 detects PDIR protein in human samples. PA3-007 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ∽58 kDa protein representing PDIR in human fibrosarcoma HT 1080 cell lysate. To a lesser extent, this antibody also detects two unknown, nonspecific bands at 47 and 30 kDa. The PA3-007 immunogen is a synthetic peptide corresponding to residues T(498) N Y I R A L R E G D H E R L G K K(515) of human PDIR. This peptide (Cat. No. PEP-226) is available for use in neutralization and control experiments.

PDIR (protein disulfide isomerase-related protein) is a novel PDI protein that was originally isolated from a human placental cDNA library. It has been suggested that from its nucleotide sequence, PDIR has three CXXC-like motifs (Cys-Ser-Met-Cys, Cys-Gly-His-Cys and Cys-Pro-His-Cys), which are found in the PDI superfamily of proteins. The CXXC motif is responsible for oxidoreductase activity. PDIR is also shown to have a putative endoplasmic reticulum (ER) retention signal ′Lys-Glu-Glu-Leu′ at its carboxyl terminus, indicative of ER resident proteins. Northern blot analysis of PDIR indicates that it is preferentially expressed in cells actively secreting proteins. It has also been shown that expression of PDIR is stress-inducible. Data suggest that PDIR, like other PDIs, has oxidoreductase activity of disulfide bonds and facilitates the protein folding process within the lumen of the ER.