Invitrogen™ SERCA2 ATPase Monoclonal Antibody (2A7-A1)

Description

MA3-919 detects sarcoplasmic or endoplasmic reticulum calcium (SERCA) 2 ATPase from canine, porcine, human, mouse, rabbit and rat tissues. MA3-919 recognizes the SERCA2a and SERCA2b isoforms identically. However, the antibody does not recognize the skeletal muscle (SERCA1) or housekeeping isoforms (SERCA3). MA3-919 has been successfully used in Western blot, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~110 kDa protein representing SERCA2 ATPase in rat cardiac tissue. The MA3-919 antigen is purified canine cardiac sarcoplasmic reticulum vesicles.

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms.