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TEV Cleavage Site Monoclonal Antibody (9A10-4C3), Invitrogen™
Mouse Monoclonal Antibody
Marque: Invitrogen MA1124
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Description
MA1-124 has been successfully used in Western blot applications. The MA1-124 immunogen is a peptide representing the cleavage site recognized by TEV enzyme.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the "e;TAP tag"e; (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.
Spécification
| TEV Cleavage Site | |
| Monoclonal | |
| 1 mg/mL | |
| PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide | |
| Mouse | |
| Protein A | |
| RUO | |
| Tag | |
| Antibody | |
| IgG1 |
| Western Blot | |
| 9A10-4C3 | |
| Unconjugated | |
| Tobacco Etch Virus | |
| KLH conjugate peptide representing the cleavage site recognized by TEV enzyme | |
| 50 μg | |
| Primary | |
| 4° C | |
| Liquid | |
| Tag |
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