PeproTech TGF alpha Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TGF-alpha. Anti-Human TGF-alpha-specific antibody was purified by affinity chromatography employing an immobilized Human TGF-alpha matrix. Sandwich ELISA: To detect Human TGF-alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TGF-alpha (500-P16Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TGF-alpha. Western Blot: To detect Human TGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human TGF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.