Vγ 3 TCR Hamster anti-Mouse, Brilliant Violet 786, Clone: 536, BD Optibuild™

Description

The 536 monoclonal antibody specifically recognizes Vγ3† T-cell Receptor (TCR)-bearing T lymphocytes, which are the predominantγδTCR-bearing cells in the early fetal thymus and the adult epidermis of euthymic mice. The first T cells to mature in the embryonic thymus express the Vγ3 and Vδ1 TCR chains, and their development is dependent upon IL-7. There is evidence that the Vγ3 TCR-bearing fetal thymocytes are the precursors of the majority of dendritic epidermal T cells (DEC), which may be replenished in the adult by proliferation in situ /rather than by seeding from primary lymphoid organs. Although the Vγ3 TCR is almost exclusively found in the DEC population, it has been shown that the homing of DEC to the epidermis does not require expression of the Vγ3 gene segment. Vγ3 TCR-bearing dermal dendritic cells have also been described. Vγ3 TCR has also been found on a subset of T lymphocytes in the lactating mammary gland and at the site of antigenic challenge in contact-sensitized mice. Plate-bound 536 antibody activates Vγ3 TCR-bearing T cells, and Fab fragments of mAb 536 block in vitro /stimulation of DEC by keratinocytes.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.