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CD137 Ligand (4-1BB Ligand) Monoclonal Antibody (4H3), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
153.00€ - 423.00€
Spécification
Antigène | CD137 Ligand (4-1BB Ligand) |
---|---|
Clone | 4H3 |
Concentration | 5 μL/Test |
Applications | Flow Cytometry |
Classification | Monoclonal |
Code produit | Marque | Quantité | Prix | Quantité et disponibilité | |||||
---|---|---|---|---|---|---|---|---|---|
Code produit | Marque | Quantité | Prix | Quantité et disponibilité | |||||
15855443
|
Affymetrix eBioscience
46-5906-42 |
100 Tests |
423.00€
100 pièces |
Expédition estimée: 17-05-2024 Connectez-vous pour voir le stock disponible |
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15845443
|
Affymetrix eBioscience
46-5906-41 |
25 Tests |
Cet article est actuellement indisponible ou discontinu.
Veuillez consulter la page produit pour de possible alternative dans l’onglet |
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Description
This 4H3 monoclonal antibody recognizes human CD137 ligand, also known as 4-1BB ligand, TNFSF9, or CDw137L. Applications Reported: This 4H3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 4H3 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. CD137L is a co-stimulatory molecule that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation. Its receptor, CD137/4-1BB, is found on a variety of cells, including inflamed endothelial cells, where its expression enhances extravasation of CD137L expressing monocytes. Activated T cells also express CD137, and engagement with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137L is also expressed on a majority of B-cell lymphomas, including mantle cell lymphoma, follicular lymphoma, and diffuse large B-cell lymphoma (but it is lacking on Hodgkin lymphoma and T-cell lymphoma). CD137L is also expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interactions.Spécification
CD137 Ligand (4-1BB Ligand) | |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
0.2%PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
41BBL, TNFRSF9, 4-1BBL, TNFSF9, CDw137L | |
TNFSF9 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
TNFSF9 |
4H3 | |
Flow Cytometry | |
PerCP-eFluor 710 | |
Mouse | |
Human | |
P41273 | |
8744 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |